Biology Forum › Molecular Biology › mrna in E coli
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- November 29, 2008 at 2:56 am #10517
crazy99Participantin a piece of hemoglobin beta chain,how will u look to see if an mRNA exists in E coli.
what am not able to understand is that am i supposed to search for mrna in e coli ‘s hemoglobin beta chain.am confused how to go about this question.
- November 29, 2008 at 4:20 am #87427MrMisteryParticipant
did you clone the beta-hemoglobin gene in E.coli?
- December 1, 2008 at 4:51 pm #87468jonmoultonParticipant
It sounds like you want to determine whether an mRNA is being expressed from a beta-globin gene in E. coli. One approach is to look for a beta-globin sequence in the total mRNA expressed by the bacterium. Here are two techniques that could be useful:
http://en.wikipedia.org/wiki/RT-PCR
http://en.wikipedia.org/wiki/Northern_blot
MrMistery asked whether the beta-hemoglobin gene was cloned into E. coli. If so, that can simplify the problem. If the gene is inserted into a plasmid vector in the E. coli, you could do a plasmid prep and so produce a less complex population of nucleic acid to probe or sequence.
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