mutation of psb

[mslynnlynn]

mutagenesis of PSB by NTG can have significant effect?

[david23]

NTG – nitrosoguanidine (N-methyl-N’-nitro-N-nitrosoguanidine – MNNG) modify bases and/or phosphates by alkylating them. The DNA becomes distorted as a result and the ability of proteins to recognize and bind correctly is hindered.

I know what NTG is, but what is PSB, not everyone is familiar with these abbreviations

[mslynnlynn]

thank u for ur reply.
psb mean phosphate solubilizing bacteria.
what method can be used to meaure the gluconic acid concentration?
how can detect the p solubilizing activity of bacteria quantitatively?

[seaseasea]

OK. I think your question is very professional and many viewers may be unfamiliar with this. I think you could search reference database to find what you need. Hope this helps.

[blcr11]

http://cat.inist.fr/?aModele=afficheN&cpsidt=1480073 measures p solubilization by clear-zone formation around colonies on agar plates (the agar medium must contain, in addition to the usual nutrients, an insoluble suspension of phosphate (CaPO4 perhaps) salts of some kind: a clear “halo” forms around colonies that are solubilizing the phosphate; how wide the halo is is a measure of the degree of p-solubilization activity).

http://www.blackwell-synergy.com/doi/ab … .tb13383.x

Comparison of two different plate (“halo”) media. Also does a quantitative solubilization assay in liquid cultures by determining the amount of CaPO4 remaining in the medium after a set incubation period using a colorimetric phosphate assay.

http://www.ncbi.nlm.nih.gov/entrez/quer … med_docsum

http://www.ncbi.nlm.nih.gov/entrez/quer … s=14506853

Two papers talking about gluconic acid and p-solubiliztion. Don’t know if you have access to these journals or not. The refs given in the paper should help find a suitable assay for gluconic acid. I would guess there’s HPLC and/or spectrophotometric/colorimetric assays. Keto-glutarate, for example, is (if I remember correctly) an orcinol-positive sugar, but then so are many sugars so if it needs to be specific, there has to be some sort of separation before quantitation.

[blcr11]

That should’ve been keto-glucuronate, not glutarate (which may or may not be orcinol-positive, but isn’t a sugar anyway).

[mslynnlynn]

thank u for ur answer.
i have problem to get pdf from ur websites.
our country cannot open full text.
so, can u help me to get full text?
my email is light.lynn@gmail.com.
thanks a lot.

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