Biology Forum › Molecular Biology › nested pcr
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- October 6, 2009 at 12:29 pm #11949
shuk08ParticipantHow to get rid of larger non-specific bands appearing in 1st round of pcr just above the expected bands (of approx. 1.5 kb)? The non-specific bands from the 1st pcr are amplified in 2nd round of pcr using nested pair of primer.
- October 6, 2009 at 2:02 pm #93380JackBeanParticipant
Are you asking or just saying us?
- October 6, 2009 at 2:19 pm #93384canalonParticipant
I would try to optimize the first rund of PCR. Tips and tricks to do thta can be found here:
http://info.med.yale.edu/genetics/ward/tavi/Guide.html - October 10, 2009 at 9:15 am #93505roniadamParticipant
I had the same problem before. I advice you to use an adjuvant to improve your PCR effeciency. I used Q solution that comes with hotstar PCR master mix and it gives me a very good amplicon.
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