Biology Forum › Human Biology › Neubaeur haemocytometer

last updated by biology_06er 18 years, 9 months ago
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    • May 5, 2006 at 12:21 am #4688
      biology_06er
      Participant

      Hi there

      I have a question that I cannot seem to do…(I have a blood lab coming up and these a few practice questions to do before hand)..could someone please help me!

      Using a Neubaeur haemocytometer, you make a count of leucocytes in a sample of human blood. In three 1 mm2 squares, you count 300 cells. What is your estimate of white blood cell density? (hint: the depth of the counting chamber is 0.1 mm).

      -9,000 cells/µL
      -1,000 cells/µL
      -100 cells/µL
      -90 cells/µL
      -900 cells/µL

      all i know is that the formula (C/V)*M is used other than that not sure c=300 v=? m=?

      thanks alot
      biology_06er

    • May 5, 2006 at 1:33 am #47898
      canalon
      Participant

      Calculate the volume of a square. You got surface and depth, this shouldn’t be too hard. Convert in Microliters.
      How many cells per square on average (divide number of cells by number of square) you then have a number of cells per volume and you convert in the right units… Et voila.

    • May 8, 2006 at 12:31 pm #48066
      biology_06er
      Participant

      Thanks-much appreciated

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