Oligo Designing Software

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    • #14357
      pushel
      Participant

      Hello,
      I am currently doing some cloning into a vector. For variouse reasons I need to order not simply sense and antisense oligos with 5′ overhangs, but sense and antisense strands that overlap over a small nucleotide sequense. After I anneal them, I polymerase them using Klenow, cut them with restriction enzymes and then clone the cut insert into the vector.
      To plan all this we use the GeneRunner software from 1994 which is quite insufficient to be honest and causes a lot of headaches and mistakes during planning.

      I wonder what is the leading software in the market for doing this type of calculations? I heared something about Oligo 7 and Vector NTI, but I am not really sure…..

      Please help ❓ ❓ ❓

      Arik

    • #103107
      magicsiew
      Participant

      Oligo 7 is good. Besides, u can try Lasergene.

    • #103108
      canalon
      Participant

      Primer3, here in only one of its incarnation, also available on NCBI) does a really good job, has a lot of parameters and can be accessed for free.

    • #103116
      pushel
      Participant

      I started looking at the SeqBuilder tool from the lasergene suite and I think this is just what I need.

      Thanks!!!

    • #103266
      nchao
      Participant

      Bio-Synthesis, Inc. provides a comprehensive list of scientific applications. Please visit the following website:
      http://www.biosyn.com/sci-apps.aspx

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