Oligo Designing Software

last updated by

nchao 13 years, 10 months ago

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- January 11, 2011 at 5:16 pm #14357
  pushel
  Participant
  Hello,
  I am currently doing some cloning into a vector. For variouse reasons I need to order not simply sense and antisense oligos with 5′ overhangs, but sense and antisense strands that overlap over a small nucleotide sequense. After I anneal them, I polymerase them using Klenow, cut them with restriction enzymes and then clone the cut insert into the vector.
  To plan all this we use the GeneRunner software from 1994 which is quite insufficient to be honest and causes a lot of headaches and mistakes during planning.
  I wonder what is the leading software in the market for doing this type of calculations? I heared something about Oligo 7 and Vector NTI, but I am not really sure…..
  Please help ❓ ❓ ❓
  Arik
- January 11, 2011 at 6:14 pm #103107
  magicsiew
  Participant
  Oligo 7 is good. Besides, u can try Lasergene.
- January 11, 2011 at 6:50 pm #103108
  canalon
  Participant
  Primer3, here in only one of its incarnation, also available on NCBI) does a really good job, has a lot of parameters and can be accessed for free.
- January 12, 2011 at 8:54 pm #103116
  pushel
  Participant
  I started looking at the SeqBuilder tool from the lasergene suite and I think this is just what I need.
  Thanks!!!
- January 20, 2011 at 6:32 pm #103266
  nchao
  Participant
  Bio-Synthesis, Inc. provides a comprehensive list of scientific applications. Please visit the following website:
  http://www.biosyn.com/sci-apps.aspx
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