Viewing 1 reply thread
  • Author
    Posts
    • #14291
      magicsiew
      Participant

      Hi, in order to save cost of RT-PCR, I was thinking to do reverse transcription from mRNA to cDNA and use that cDNA in PCR for large scale screening purpose. I would like to ask will these two reaction give same results? Both RT-PCR using RNA and PCR using cDNA. Thank you.

    • #102901
      Nicky2010
      Participant

      Hi
      I think these two reaction can give the same results.
      Because RT-PCR refers to reverse transcription PCR and include "mRNA to cDNA" and "c
      DNA to dsDNA.
      In fact, doing reverse transcription from mRNA to cDNA yourself is one step of RT-PCR. they are the same.

Viewing 1 reply thread
  • You must be logged in to reply to this topic.