Biology Forum › Molecular Biology › PCR
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- March 12, 2011 at 12:37 am #14638
sarah111ParticipantHi im abit confussed and would really appreciate if someone could help me with this.
The project objective is to obtain actin gene from genomic DNA, insert amplicon into a plasmid and confirm with qRTPCR.
How exactly would i go about doing this? - March 12, 2011 at 8:54 am #103870JackBeanParticipant
find your gene e.g. at PubMed or some other specialized database (depends on your organism), then you need to design primers for that sequence, best with some restriction places. Cut the amplicon and vector with RE. Mix and ligate.
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