PCR - Biology Forum

last updated by

canalon 16 years, 6 months ago

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- May 14, 2008 at 11:18 am #9638
  shivakumar
  Participant
  hi everybody
  In order to study the role of some genes, I have designed gene specific primers and made a PCR. And I separated the amplified products in agarose gel and got bands. Surprisingly I got the same bands with the PCR product which was done without using cDNA template(which was for the confirmation of amplification). Can anybody answer me, how will get band even without template.
- May 14, 2008 at 1:10 pm #84103
  canalon
  Participant
  the first answer tha comes to mind is: contamination.
  Now the proble is with what and how. This implies using new unopened reagents (or old reagents that cannot have been contaminated), decontaminating pipettes and/or using filter tips and rethinking you good lab practice to see how you have meade the contamination possible so as to avoid it next time.
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