Biology Forum Molecular Biology Primer design

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    • #15849

      Hi, first of all guys, i do not expect you guys to do my work for me, i just would like a clearer understanding of what i am suppose to learn or direction toward what i should be looking at. I have been given a computer based assignment regarding primer design. I have found my nucleotide sequence, ran them through the yeast genome software and it came up with a set of primers. Now i have to calculate the approximate length of the amplified sequence. with the following criteria to be noted : housekeeping gene has to be amplified at the same time, same sample etc, target mRNA should produce a DNA distingushable with housekepper and taq polymerase adds 100 bp per min.

      I have been able to do 80% of the task, i am confused as to how i calculate the lenght of the amplified sequence, do i substract the starting point of the primers (offset) from the total size of the selected sequence?

      Any help or reply would be highly appreciated


    • #108769

      length of amplicon = position of rev primer – position of fw primer

    • #108801

      you should use some software to count it, example: BioEdit…

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