Biology Forum Molecular Biology primer design for DNA through mRNA sequence

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    • #9718
      ss_kalve
      Participant

      Hi,
      Can anybody tell that through availability of mRNA sequence can we design primer for genomic DNA. which kind of software we can use?

    • #84455
      Cat
      Participant

      Yes, any. ❓

    • #84961
      biznatch
      Participant

      Yes you can use the mRNA sequence to design gDNA primers. If the primers happen to span an intron (one on each side of the intron) the product from gDNA will be larger then from mRNA and if the intron is very big you might not be able to span its length by PCR. I use Primer3 (http://fokker.wi.mit.edu/cgi-bin/primer … r3_www.cgi) for designing primers.

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