Biology Forum › Molecular Biology › primer design for DNA through mRNA sequence
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- June 4, 2008 at 5:02 am #9718ss_kalveParticipant
Hi,
Can anybody tell that through availability of mRNA sequence can we design primer for genomic DNA. which kind of software we can use? - June 4, 2008 at 11:38 pm #84455CatParticipant
Yes, any. ❓
- July 7, 2008 at 10:29 pm #84961biznatchParticipant
Yes you can use the mRNA sequence to design gDNA primers. If the primers happen to span an intron (one on each side of the intron) the product from gDNA will be larger then from mRNA and if the intron is very big you might not be able to span its length by PCR. I use Primer3 (http://fokker.wi.mit.edu/cgi-bin/primer … r3_www.cgi) for designing primers.
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