primer design for DNA through mRNA sequence - Biology Forum

last updated by

biznatch 16 years, 7 months ago

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- June 4, 2008 at 5:02 am #9718
  ss_kalve
  Participant
  Hi,
  Can anybody tell that through availability of mRNA sequence can we design primer for genomic DNA. which kind of software we can use?
- June 4, 2008 at 11:38 pm #84455
  Cat
  Participant
  Yes, any. ❓
- July 7, 2008 at 10:29 pm #84961
  biznatch
  Participant
  Yes you can use the mRNA sequence to design gDNA primers. If the primers happen to span an intron (one on each side of the intron) the product from gDNA will be larger then from mRNA and if the intron is very big you might not be able to span its length by PCR. I use Primer3 (http://fokker.wi.mit.edu/cgi-bin/primer … r3_www.cgi) for designing primers.
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