Protein expression - Biology Forum

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Cat 16 years, 8 months ago

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- March 20, 2008 at 1:51 pm #9320
  roniadam
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  HI
  Can anyone tell me why is the frame of the inserted DNA important in bacterial expression system while it is not important for mammalian and baculovirus system?
- March 20, 2008 at 2:45 pm #82952
  Cat
  Participant
  I am not sure I understand your question. DNA inserted where – into a transformation vector or host genome? Also, what is the insert that it has to be in frame? ORF without ATG? You see in most bacterial expression systems your plasmid transformation vector is usually transcribed directly…
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