protein gel - Biology Forum

last updated by

JackBean 15 years, 1 month ago

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- October 28, 2009 at 12:13 pm #12126
  piefke
  Participant
  Hi everybody!
  I want to check the cleavage of a protein. The fragments I expect are 54 kDa and 8 kDa…
  8 kDa is very small 🙁 Is it possible to see this small thing on a sds-gel??? 😯
- October 28, 2009 at 1:10 pm #94166
  JackBean
  Participant
  Sure, why not? You just have to choose the right concentration of AA/BIS (maybe use gradient, to have good resolution at both 8 and 54 kDa;), use silver staining and maybe add a little bit more of protein to see it 😉
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