Biology Forum › Microbiology › Question about Kirby-Bauer method
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- February 10, 2012 at 5:15 am #16064
miramaxParticipantHi, I’m trying to do the Kirby-Bauer method by testing the effect of certain foods’ ability to inhibit pathogen growth.
I was wondering if it’s possible to do this method by just streaking the agar plates with pathogens like E.Coli straight from the tube (from the catalogue) or is it a must to use only pathogens after it has been grown in broth??
My plan was to just streak like E. Coli with an inoculating loop in a zig-zag pattern every 90 degrees, pretty much covering the whole plate. And then I would place my paper disks on top of the agar…
Thanks for replies.
- February 10, 2012 at 7:41 am #109567JackBeanParticipant
Usually you do overnight culture first. First, you will make sure the bacteria are viable. Second, you don’t want to run out of your stock and order it again 😉
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