Random priming method - Biology Forum

last updated by

DevGrp 18 years, 11 months ago

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- October 7, 2005 at 12:07 pm #2109
  ak537
  Participant
  Can any one write all possible(its 6 , I guess 🙄 ) combinations of primers in random priming?
- October 7, 2005 at 1:06 pm #30574
  canalon
  Participant
  quote ak537:
  Can any one write all possible(its 6 , I guess 🙄 ) combinations of primers in random priming?
  No, Nobody will. Not because of the ‘homework policy’ of the forum, or because we don’t want to hel you but because you made a little mistake.
  Random priming is made with hexanucleotides, or 6 nucleotide long primers with random nucleotide at each position. Hence there isn’t 6 combinations but 4^6 or 4096, and that is too much to type here 😉
  HTH
  Patrick
- October 8, 2005 at 12:06 pm #30635
  ak537
  Participant
  Thanks for this information. my concept is now clear about this and not even I wanna write all combinations 😀 😀
- October 12, 2005 at 2:15 pm #30825
  DevGrp
  Participant
  how about
  NNNNNN
  or occasionally
  NNNNNNN
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