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    • #2109
      ak537
      Participant

      Can any one write all possible(its 6 , I guess 🙄 ) combinations of primers in random priming?

    • #30574
      canalon
      Participant
      quote ak537:

      Can any one write all possible(its 6 , I guess 🙄 ) combinations of primers in random priming?

      No, Nobody will. Not because of the ‘homework policy’ of the forum, or because we don’t want to hel you but because you made a little mistake.
      Random priming is made with hexanucleotides, or 6 nucleotide long primers with random nucleotide at each position. Hence there isn’t 6 combinations but 4^6 or 4096, and that is too much to type here 😉

      HTH

      Patrick

    • #30635
      ak537
      Participant

      Thanks for this information. my concept is now clear about this and not even I wanna write all combinations 😀 😀

    • #30825
      DevGrp
      Participant

      how about

      NNNNNN

      or occasionally

      NNNNNNN

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