reg PCR - Biology Forum

last updated by

blcr11 16 years, 10 months ago

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- February 5, 2008 at 12:27 am #9060
  nityasingh
  Participant
  i have a gene of interest that has been cloned into a plasmid. but i now need to delete certain segments of that gene & express the protein. is there a protocol available for doing so? i am trying to find any paper which contains such a protocol but am not able to find any. any help will be much appreciated.
- February 8, 2008 at 4:07 pm #81618
  blcr11
  Participant
  I don’t know what to suggest without knowing more details. These are internal sequences you need to delete? like you want to remove a specific loop of protein or something like that? Check out Stratagene. They have a range of mutagenesis kits thay may work for you.
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