Yeah if it would have been same length then it would have been easy to make map….that’s what is confusing me.
Isolated gene from an EcoR I digest contains a single Asp 1 restriction site but no other restriction sites.The gene is inserted into a plasmid digested with Ecor 1 and the plasmid with the insert selected,grown up and plasmid isolated.A probe prepared by labeling the original gene is used to analyze southern blots of three different restriction digest.prepare a map of the insert region containing the gene and explain southern blot data?
Asp 1+Ecor 1——1.6,.4
I tried to solve this..I need to make a linear map…..
The Asp1 alone digest looks to be impossible to me. You should see only two bands. They most likely won’t be the same as the EcoRI + AspI double-digest as the band lengths will be determined by how far the AspI site is from the nearest vector-derived AspI site(s). The only bands you should see are the AspI-AspI fragments that carry either the 0.4 or 1.6 kb portion of the EcoRI band. You can’t see four bands unless this is a partial digest; you should see two bands, and you may only see 1 band if the AspI gods are against you.