I just discovered that the QIAGEN RNeasy protocol says you can substitute DTT for B-Me in Buffer RLT for the elimination of RNases, but this only applies to isolation of RNA from animal tissue, not animal cells, yeast, plants, etc. This would be great since we use animal tissue, and it would mean we don’t have to do the first few steps in the hood. Does anyone know if there are any drawback to using DTT instead of B-Me, and why this substitution apparently only applies to animal tissue?
thanks!