Biology Forum › Genetics › SDS
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- January 27, 2008 at 3:08 pm #9006
okmedcuiParticipantHi,
During the experiment of extraction of genomic DNA from E.coli, we added 2% SDS/2N NaOH solution to a cell suspension containing lysozyme. What’s the use of SDS?
thx - January 27, 2008 at 4:10 pm #81190mithParticipant
look it up
- January 27, 2008 at 4:20 pm #81192okmedcuiParticipant
I understand that it denatures proteins.
but bacteria were lysed by lysozyme and lysis buffer, why need sds? - January 27, 2008 at 4:53 pm #81193mithParticipant
http://www.bio.davidson.edu/COURSES/GEN … SPAGE.html
- January 28, 2008 at 9:45 am #81201okmedcuiParticipant
Thank u!
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