Biology Forum › Genetics › SDS

last updated by okmedcui 16 years, 3 months ago
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    • January 27, 2008 at 3:08 pm #9006
      okmedcui
      Participant

      Hi,
      During the experiment of extraction of genomic DNA from E.coli, we added 2% SDS/2N NaOH solution to a cell suspension containing lysozyme. What’s the use of SDS?
      thx

    • January 27, 2008 at 4:10 pm #81190
      mith
      Participant

      look it up

    • January 27, 2008 at 4:20 pm #81192
      okmedcui
      Participant

      I understand that it denatures proteins.
      but bacteria were lysed by lysozyme and lysis buffer, why need sds?

    • January 27, 2008 at 4:53 pm #81193
      mith
      Participant

      http://www.bio.davidson.edu/COURSES/GEN … SPAGE.html

    • January 28, 2008 at 9:45 am #81201
      okmedcui
      Participant

      Thank u!

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