Biology Forum › Molecular Biology › SDS problem

last updated by roman 15 years ago
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    • June 27, 2009 at 8:48 am #11501
      smallrat
      Participant

      the dying protein appear a distrubution cuve when I run the gel ..

      I do see a buble in the middle of my gel, but I wonder why it happen..

      because bubbles in the champer or there is gaps between the acrylic pressure plate and the longer glass plate?

      any idea ?
      thanks

    • June 27, 2009 at 10:58 am #91592
      alicejaya
      Participant

      hi
      I think ur gel has already statrted solidifying before you started pouring the gel.
      So carefully follow the proceedure

    • November 4, 2009 at 7:45 am #94493
      roman
      Participant

      Hello , I think you must pour the gel quickly after all the reagents were added.

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