Biology Forum › Molecular Biology › seperate nucleotides by electrophoresis
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- December 30, 2005 at 12:53 pm #3044
goose_keeperParticipantwhat is the best pH value when seperating AMP UMP CMP GMP in electrophoresis?
Thanks for your attention!
- December 30, 2005 at 6:56 pm #35857JumpshooterParticipant
to separate those nucleotides. Try using Tris EDTA buffer adjusted to pH = 6.25 as the mobile phase on your agarose gel.
- December 31, 2005 at 12:57 am #35881jeffreyParticipant
“Nucleic acids however, remain negative at any pH used for electrophoresis and in addition carry a fixed negative charge per unit length of molecule, provided by the PO4 group of each nucleotide of the the nucleic acid. Electrophoretic separation of nucleic acids therefore is strictly according to size.”
so, my opinon is just give a PH which can not destroy these nucleotides - December 31, 2005 at 4:10 am #35891goose_keeperParticipant
pH A, 1.5 B, 3.5
C, 6.5 D, 9.5
I don’t know why and which one I should choose .
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