seperate nucleotides by electrophoresis

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    • #3044
      goose_keeper
      Participant

      what is the best pH value when seperating AMP UMP CMP GMP in electrophoresis?

      Thanks for your attention!

    • #35857
      Jumpshooter
      Participant

      to separate those nucleotides. Try using Tris EDTA buffer adjusted to pH = 6.25 as the mobile phase on your agarose gel.

    • #35881
      jeffrey
      Participant

      “Nucleic acids however, remain negative at any pH used for electrophoresis and in addition carry a fixed negative charge per unit length of molecule, provided by the PO4 group of each nucleotide of the the nucleic acid. Electrophoretic separation of nucleic acids therefore is strictly according to size.”
      so, my opinon is just give a PH which can not destroy these nucleotides

    • #35891
      goose_keeper
      Participant

      pH A, 1.5 B, 3.5
      C, 6.5 D, 9.5
      I don’t know why and which one I should choose .

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