sequencing

December 9, 2010 at 3:48 pm #14256

Participant

hi friends,
I know some of methods for Sanger dna sequencing, maxam gilbert sequencing, pyrosequencing, automated dna sequencing which are used for dna sequencing. But I don’t know how for these method sequences dna?

December 9, 2010 at 4:14 pm #102692

JackBean

Participant

I’m sorry, but what are you asking for?

December 9, 2010 at 5:40 pm #102694

magicsiew

Participant

All the methods u stated above involve complicated steps, not really suitable u ask people to explain all the detail from forum. I will recommend you to study each of them 1st, how they works to sequence the DNA, if you have any doubt on some of the steps, then u post your question here, I am sure many people will be willing to help you here… ^^

December 9, 2010 at 7:03 pm #102697

canalon

Participant

Actually if you go on Wikipedia, all those methods are extensively and correctly explained (except, as far as I know, the automated which is just an extension of the Sanger or pyrosequencing one, but automated to save human labour).

December 10, 2010 at 3:53 pm #102714

kanagasundar

Participant

hi to all,
Thanks. I know steps in each DNA sequencing method. I need to know how much size of DNA these method can be read?

"Like the automated sequencing method is only accurate for sequences up to a maximum of about 700-800 base-pairs in length"

December 10, 2010 at 4:22 pm #102717

canalon

Participant

The different methods of next-gen sequencing
Illumina and SOLiD are about 35 to 50bp
454 pyrosequencing claims to attain 400 to 500 bp and that it will be able to reach 1000bp
Pacific bioscience claims that it will be able to go up to 1500bp with helicase based 3rd gen sequencing

Sanger based will give easily 700-800bp read in an experienced lab, 500bp if you are a DIY, and up to 1500bp if all parameters can be optimized (usually that is not automated).

December 11, 2010 at 1:27 pm #102724

kanagasundar

Participant

Thank u canalon

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