SONICATION OF DNA - Biology Forum

last updated by

canalon 13 years, 11 months ago

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5 replies

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- November 17, 2010 at 11:19 pm #14145
  bill1
  Participant
  Heya,
  Could you help me with this subject? I need to find out how long do I need to sonicate DNA? any suggestions any publications??
  I really appreciate your help 🙂
- November 18, 2010 at 1:11 am #102325
  canalon
  Participant
  Have you tried Google? lots of protocols available for free.
- November 18, 2010 at 6:51 am #102333
  JackBean
  Participant
  why do you want to sonicate DNA?
- November 18, 2010 at 1:56 pm #102349
  canalon
  Participant
  To shear it in small and relatively constant fragment before say cloning. You can also use a fine needle or a nebulizer. it is cheaper and less sequence specific than restriction enzyme, with break point that should be entirely random, which should give a better chance to get complete coverage for sequence, or to avoid cutting in the middle of your gene of interest.
- November 24, 2010 at 7:47 pm #102449
  bill1
  Participant
  Thank you for all replies. Unfortunately I wasn’t able to find as many protocols about sonication as I want:(
  If anyone of you has knowledge or links about it I would really appreciate it.
- November 24, 2010 at 7:54 pm #102450
  canalon
  Participant
  Things like:
  http://www.kreatech.com/Default.aspx?tabid=119
  http://www.genome.ou.edu/protocol_book/ … artII.html
  First and 5th link in google for DNA sonication. I fail to see what more you would need.
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