- August 23, 2006 at 6:23 am #5547
Anyone know where I can get detailed information about synthetic substrates from? Something along the lines of their uses, purposes, history etc?
Any journals or websites would be really helpful.
- August 23, 2006 at 6:25 am #53792
Give me an example and I’ll try and help you
- August 23, 2006 at 6:34 am #53795
Well, anything from as broad as what they are and their purposes, to examples such as chromogenic or fluorogenic substrates for hydrolases/glycosidases.
- August 23, 2006 at 7:22 am #53800
- August 23, 2006 at 7:35 am #53801
Seriously – I need like a general overview. No details about their synthesis or one particular substrate or type of substrate. Just a general overview.
Perhaps something about their applications, and future prospects? Their use in clinical diagnosis?
PS. I don’t know if you know, but that link doesn’t work. Thanks for your help anyway.
- August 23, 2006 at 11:07 pm #53854oppoxParticipant
yea I think he knew 😉 . I think the best u can do is search it, search googles and in journals etc. I dont think u will find an overview in one place since its alot of substrates for alot of different proteins.
- August 24, 2006 at 9:02 pm #53876
- August 25, 2006 at 1:03 am #53883
Thank you oppox for your reply 🙂 I have tried google and journals, but can’t really find much about where it all started and who came up with the idea and why – but I shall keep looking anyway! I need to write an introduction on "synthetic enzyme substrates and their applications". More specifically, I will need to focus on chromogenic substrates for glycosidases. Thanks again – I’ll keep looking 🙂
Weesper – lol…I can handle a joke – I just didn’t know it was one 🙂
So perhaps something like http://www.isitajoke?.com would be more uselful for me. I can click on the FAQ page and read up the "50 ways of determining when someone is being funny over the internet" section…
thanks anyway! 🙂
- August 25, 2006 at 3:21 am #53885canalonParticipant
As far as I remember it started with Monod and the use of IPTG/Xgal to study the Beta-galactosidase activity. But it was long ago and I do not have the book anymore 🙁
- August 25, 2006 at 7:03 pm #53906
Thank you for the information – it’s helpful. I’ll try searching that as well 🙂
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