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    • #5547
      felicity_wishes
      Participant

      Anyone know where I can get detailed information about synthetic substrates from? Something along the lines of their uses, purposes, history etc?

      Any journals or websites would be really helpful.

      Many thanks.

    • #53792
      weesper
      Participant

      Give me an example and I’ll try and help you

    • #53795
      felicity_wishes
      Participant

      Well, anything from as broad as what they are and their purposes, to examples such as chromogenic or fluorogenic substrates for hydrolases/glycosidases.

      Thank you.

    • #53800
      weesper
      Participant

      Oh so you want to everything?

      Go to http://www.syntheticsubstrates.com

      Seriously if you’re asking such as broad question I can only give you broad replies. Give me one concrete example.

    • #53801
      felicity_wishes
      Participant

      Seriously – I need like a general overview. No details about their synthesis or one particular substrate or type of substrate. Just a general overview.

      Perhaps something about their applications, and future prospects? Their use in clinical diagnosis?

      PS. I don’t know if you know, but that link doesn’t work. Thanks for your help anyway.

    • #53854
      oppox
      Participant

      yea I think he knew 😉 . I think the best u can do is search it, search googles and in journals etc. I dont think u will find an overview in one place since its alot of substrates for alot of different proteins.

    • #53876
      weesper
      Participant

      Yes next time I’ll also put a link up to http://www.canyouhandleajoke.com to make sure the message gets across. take care good luck

    • #53883
      felicity_wishes
      Participant

      Thank you oppox for your reply 🙂 I have tried google and journals, but can’t really find much about where it all started and who came up with the idea and why – but I shall keep looking anyway! I need to write an introduction on "synthetic enzyme substrates and their applications". More specifically, I will need to focus on chromogenic substrates for glycosidases. Thanks again – I’ll keep looking 🙂

      Weesper – lol…I can handle a joke – I just didn’t know it was one 🙂
      So perhaps something like http://www.isitajoke?.com would be more uselful for me. I can click on the FAQ page and read up the "50 ways of determining when someone is being funny over the internet" section…
      thanks anyway! 🙂

    • #53885
      canalon
      Participant

      As far as I remember it started with Monod and the use of IPTG/Xgal to study the Beta-galactosidase activity. But it was long ago and I do not have the book anymore 🙁

    • #53906
      felicity_wishes
      Participant

      Thank you for the information – it’s helpful. I’ll try searching that as well 🙂

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