thermus aquaticus

October 8, 2007 at 11:45 pm #8350

Participant

Ok.. so how do the enzymes of this bacteria compare with other microorganisms’ enzymes?? I know they are function at high temp and reproduce very fast! but what else?? How might they prove to be valuable in the future? Any help appreciated. I have been searching online forever.

October 8, 2007 at 11:50 pm #76736

mith

Participant

pcr

October 9, 2007 at 4:54 pm #76747

BDeis

Participant

As mentioned above, the dna polymerase of this org. is used in pcr. Hence, the Taq bead. Since they live in high temps the polymerase can handle the high denaturing temps of pcr. If you were using mesophilic proteins they would denature during each cycle. This would leave you a big gooey mess and no dna amplification. That is the current use. As for future uses I don’t know. Perhaps you can expand on future uses of pcr technology.

October 9, 2007 at 5:14 pm #76750

mith

Participant

I just learnt this today, pcr was invented by a surfer dude. Rad!

October 10, 2007 at 12:21 pm #76768

MrMistery

Participant

karry mullis or the guy he stole the idea from?

October 20, 2007 at 5:30 am #77035

victor

Participant

Karry Mullis is a surfer? wow….
I also wondering, what force that makes Taq polymerase can withstand high temperature? how can it’s 3D structure can does that….

October 20, 2007 at 7:06 am #77040

Jammerz

Participant

Probably a large number of S-S disulfide bonds in addition to much hydrogen bonding. But I’m only guessing, don’t quote me on that.

October 20, 2007 at 7:33 am #77041

MrMistery

Participant

I was gonna make exact the same guess 😆

October 22, 2007 at 9:27 am #77107

victor

Participant

thanks for the informations boss… 😀
hmm….there must be another else that makes this thing soooo strong 😕
I really curious about those force….

October 22, 2007 at 8:20 pm #77120

MrMistery

Participant

http://www.rcsb.org/pdb/explore.do?structureId=1TAQ
Here, download the structure and explore it. I do not have Chimera on this computer, but you should be able to deduce by analysing it with Chimera what are the main bonds.

October 22, 2007 at 8:40 pm #77123

mith

Participant

chimera is free 🙂

October 23, 2007 at 9:45 am #77135

MrMistery

Participant

i know, but this is my sister’s laptop and she has a strict "No biochemistry geekish stuff on my laptop" rule

October 26, 2007 at 9:51 am #77215

victor

Participant

😆 she ‘s afraid that she’ll be turned like you Andrew 😆

October 28, 2007 at 10:20 pm #77279

victor

Participant

AARRRGGGHHHH…..!!! NOOOO….!!!
I have just downloaded the Chimera program and started to run it. But I was confused that the preface of this program is a little bit kinda crash I think….
But when I read the system requirements, it states that, "even that your computer works well with Quake game, it doesn’t mean that it will work well also with this scientific program such as Chimera"
🙁 🙁 🙁

My laptop even can’t play Quake, then how can it run Chimera…. 🙁