Biology Forum Microbiology thermus aquaticus

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    • #8350
      aztectgirl
      Participant

      Ok.. so how do the enzymes of this bacteria compare with other microorganisms’ enzymes?? I know they are function at high temp and reproduce very fast! but what else?? How might they prove to be valuable in the future? Any help appreciated. I have been searching online forever.

    • #76736
      mith
      Participant

      pcr

    • #76747
      BDeis
      Participant

      As mentioned above, the dna polymerase of this org. is used in pcr. Hence, the Taq bead. Since they live in high temps the polymerase can handle the high denaturing temps of pcr. If you were using mesophilic proteins they would denature during each cycle. This would leave you a big gooey mess and no dna amplification. That is the current use. As for future uses I don’t know. Perhaps you can expand on future uses of pcr technology.

    • #76750
      mith
      Participant

      I just learnt this today, pcr was invented by a surfer dude. Rad!

    • #76768
      MrMistery
      Participant

      karry mullis or the guy he stole the idea from?

    • #77035
      victor
      Participant

      Karry Mullis is a surfer? wow….
      I also wondering, what force that makes Taq polymerase can withstand high temperature? how can it’s 3D structure can does that….

    • #77040
      Jammerz
      Participant

      Probably a large number of S-S disulfide bonds in addition to much hydrogen bonding. But I’m only guessing, don’t quote me on that.

    • #77041
      MrMistery
      Participant

      I was gonna make exact the same guess 😆

    • #77107
      victor
      Participant

      thanks for the informations boss… 😀
      hmm….there must be another else that makes this thing soooo strong 😕
      I really curious about those force….

    • #77120
      MrMistery
      Participant

      http://www.rcsb.org/pdb/explore.do?structureId=1TAQ
      Here, download the structure and explore it. I do not have Chimera on this computer, but you should be able to deduce by analysing it with Chimera what are the main bonds.

    • #77123
      mith
      Participant

      chimera is free 🙂

    • #77135
      MrMistery
      Participant

      i know, but this is my sister’s laptop and she has a strict "No biochemistry geekish stuff on my laptop" rule

    • #77215
      victor
      Participant

      😆 she ‘s afraid that she’ll be turned like you Andrew 😆

    • #77279
      victor
      Participant

      AARRRGGGHHHH…..!!! NOOOO….!!!
      I have just downloaded the Chimera program and started to run it. But I was confused that the preface of this program is a little bit kinda crash I think….
      But when I read the system requirements, it states that, "even that your computer works well with Quake game, it doesn’t mean that it will work well also with this scientific program such as Chimera"
      🙁 🙁 🙁

      My laptop even can’t play Quake, then how can it run Chimera…. 🙁

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