This may be a very simple question but I just wanted to confirm that the transcription of the plasmid DNA occurs in cytoplasm in transient transfection of mammalian cells. I know that for stable transfection, the gene of interest should be integrated into the host’s chromosome, but in transient transfection, does the transcription of the gene of interest occur without the need of plasmid actually entering into the nucleus?
I also thought about this problem.
In the transient transfection, where do the plasmids go , the nucleus, or the cytosol?
If it is mainly in the cytosol,is it possible that there is mechanism of the cytoplasmic transcription?