Triton X-100 extraction - Biology Forum

last updated by

JackBean 14 years, 8 months ago

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- February 24, 2010 at 2:31 pm #12832
  kk
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  Someone please, what does it imply if a cytoskeletal protein is removed/retained after a mild Triton X-100 wash?
  Seems to be a common technique yet no simple interpretaion of the results obtained from using this technique…
- February 24, 2010 at 3:02 pm #97891
  JackBean
  Participant
  Triton is detergent, it’s supposed to cover the hydrophobic pathes of transmembrane proteins or proteins stacked together by them. It should also break the membranes efficiently.
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