Biology Forum › Molecular Biology › TRIzol
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- May 24, 2006 at 7:25 pm #4880HELISAParticipant
Has anyone used TRIzol Reagen for total RNA extraction from plant material?
What makes this reagent so special, that it allows to avoid RNA degradation? - May 26, 2006 at 2:08 am #48984LilKimParticipant
i’ve used it a hundred millions of time for total nucleic acid extraction of mammalian cells. (and i know of people who have used it for plants)
After extraction i would DNAse treat the sample to eat the DNA and keep RNA
but that’s all I know…
– kim
- May 26, 2006 at 5:45 pm #49024HELISAParticipant
Hmm…Still, I’m waiting for some good explanation, why TRIzol is worth buying.
- May 26, 2006 at 8:19 pm #49045canalonParticipant
I went at Sigma, and saerched for trizol. And I found 3 references, including 2 in a free acces journal.
So May I suggest you to do your own research rather than waiting for people to do it for you? - May 28, 2006 at 5:01 pm #49133HELISAParticipant
Nice to meet you too…
- May 28, 2006 at 8:07 pm #49142canalonParticipantquote HELISA:Hmm…Still, I’m waiting for some good explanation, why TRIzol is worth buying.
As if that was nice. Asking a question is OK, complaining becasue you still do not have an answer is not. We are not at your service.
I was trying to give you some leads to find an answer to your question since I do not know the answer. Now I don’t have time to do all your work. - May 28, 2006 at 10:02 pm #49148LilKimParticipant
Canalon … you’re great! (… but i’m sure you already knew that…!)
- June 3, 2006 at 4:05 pm #49429tranjoParticipant
Trizol is worth buying because it’s a standard for RNA work.
Best, J
- June 3, 2006 at 5:26 pm #494342810712Participant
How does trizol look like? I’m curious but its far away from my prescribed syllabus presently…still interested…
It extracts DNA & RNA…from both animal & plant…hehe i’m too litle here!
Is sigma some kind of Database?
Ved - June 4, 2006 at 3:23 am #49445canalonParticipant
trizol is a liquid, actually a solvent used in nucleic acids extractions.
And Sigma is one of the biggest manufacturer of fine chemicals for (life) sciences. So their catalogue is a very good start to find info on many chemicals. - June 4, 2006 at 4:44 am #49451tranjoParticipant
Yes Sigma has a nice feature on the upper right column of each product page. The links include PDFs for uses, inserts, and MSDS. It’s a good place to learn about the various chemicals that could end your life!
Best, J
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