Biology Forum › Microbiology › Unknown bacteria
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- November 23, 2009 at 12:39 am #12334
HmicroParticipantI am very confused and becoming upset. I NEED HELP
These are my test results can someone give me an idea of what it could be?
Gram +
Cocci
Sucrose +
Glucose +
Lactose +
Mannitol +
Mehtyl Red +
VP test +
Catalase –
Starch –
casein –
fat hydrolysis +
urea –
kligers iron –
citrate-
phenylalanine –
litmus milk- acid reaction
What can this be please please help - November 23, 2009 at 2:57 am #95232azarulezParticipant
why don’t you give a try using API kit? it’s more easier to identify unknown bacteria..
- November 23, 2009 at 3:07 am #95235HmicroParticipant
What is a API kit?
- November 23, 2009 at 3:24 am #95239azarulezParticipantquote Hmicro:What is a API kit?
API strips give accurate identifications based on extensive databases and are standardized, easy-to-use test systems. The kits include strips that contain up to 20 miniature biochemical tests. All are quick, safe and easy to perform.
link: http://www.biomerieux-usa.com/servlet/s … _PRD_USA_5
- November 23, 2009 at 11:14 pm #95259HmicroParticipant
Okay i think i found my unknown bacteria. Now i have to write a conclusion based on my test. I don’t know how to begin this. Can someone help me . Based on my test results i think i can confirm its Enterocccus F. HELP this report is due in a 2 days 🙁
- November 23, 2009 at 11:41 pm #95260HmicroParticipant
Does it ferment Glucose what happens with methyl red and VP Test. Does hydrolysis starch, casein, fat, urea, Klingers iron?
- November 26, 2009 at 6:21 pm #95364MrMisteryParticipant
Please post all your questions regarding the same thing into the same thread, do not start a new topic every time.
- November 29, 2009 at 9:49 pm #95504HmicroParticipant
It ferments glucose, +for VP + for methyl red test, does not hydrolysis starch, casein yes, fat yes and urea no, klingers iron no
- November 30, 2009 at 2:15 am #95515HmicroParticipant
What happens with Enteroc. Fac. with
MP
VP
Casein
Fat
Urea
Kliglers Iron
Citrate
Phelalkine - November 30, 2009 at 2:44 am #95518JackBeanParticipant
what about some rRNA or tRNA or anything sequencing? It’s faster and more accurate 😉
- November 30, 2009 at 3:12 am #95523HmicroParticipant
That is not part of our lab testing. I think my unknown is E. faecalis. I just need help finding out if it is postive for methyl red and VP also if it hydrolysis exoenzymes like statch, casein, fat, tryptophan,urea, kiligers iron, citrate and phenalalkine
- December 1, 2009 at 3:30 am #95568billwParticipant
I’m in the process of writing a bacteria matching program, which you might find helpful. (And it would also help me out to have someone give it a try.)
http://moclips.net/microti/microti.php
It only has about 50 bacteria in the database at the moment, but it does give E. faecalis as the best match based on the info you have given. You can drill down to comparisons between the information you enter and the bacteria that appear to be the closest matches (which is mostly info from Bergeys, but is at least all in one place).
- December 1, 2009 at 11:30 am #95576HmicroParticipant
wow what a wonderful website. Thank you sooooooo much!
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