Biology Forum Microbiology Unknown bacteria

last updated by Hmicro 13 years ago
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12 replies
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    • #12334
      Hmicro
      Participant

      I am very confused and becoming upset. I NEED HELP
      These are my test results can someone give me an idea of what it could be?
      Gram +
      Cocci
      Sucrose +
      Glucose +
      Lactose +
      Mannitol +
      Mehtyl Red +
      VP test +
      Catalase –
      Starch –
      casein –
      fat hydrolysis +
      urea –
      kligers iron –
      citrate-
      phenylalanine –
      litmus milk- acid reaction
      What can this be please please help

    • #95232
      azarulez
      Participant

      why don’t you give a try using API kit? it’s more easier to identify unknown bacteria..

    • #95235
      Hmicro
      Participant

      What is a API kit?

    • #95239
      azarulez
      Participant
      quote Hmicro:

      What is a API kit?

      API strips give accurate identifications based on extensive databases and are standardized, easy-to-use test systems. The kits include strips that contain up to 20 miniature biochemical tests. All are quick, safe and easy to perform.

      link: http://www.biomerieux-usa.com/servlet/s … _PRD_USA_5

    • #95259
      Hmicro
      Participant

      Okay i think i found my unknown bacteria. Now i have to write a conclusion based on my test. I don’t know how to begin this. Can someone help me . Based on my test results i think i can confirm its Enterocccus F. HELP this report is due in a 2 days 🙁

    • #95260
      Hmicro
      Participant

      Does it ferment Glucose what happens with methyl red and VP Test. Does hydrolysis starch, casein, fat, urea, Klingers iron?

    • #95364
      MrMistery
      Participant

      Please post all your questions regarding the same thing into the same thread, do not start a new topic every time.

    • #95504
      Hmicro
      Participant

      It ferments glucose, +for VP + for methyl red test, does not hydrolysis starch, casein yes, fat yes and urea no, klingers iron no

    • #95515
      Hmicro
      Participant

      What happens with Enteroc. Fac. with
      MP
      VP
      Casein
      Fat
      Urea
      Kliglers Iron
      Citrate
      Phelalkine

    • #95518
      JackBean
      Participant

      what about some rRNA or tRNA or anything sequencing? It’s faster and more accurate 😉

    • #95523
      Hmicro
      Participant

      That is not part of our lab testing. I think my unknown is E. faecalis. I just need help finding out if it is postive for methyl red and VP also if it hydrolysis exoenzymes like statch, casein, fat, tryptophan,urea, kiligers iron, citrate and phenalalkine

    • #95568
      billw
      Participant

      I’m in the process of writing a bacteria matching program, which you might find helpful. (And it would also help me out to have someone give it a try.)

      http://moclips.net/microti/microti.php

      It only has about 50 bacteria in the database at the moment, but it does give E. faecalis as the best match based on the info you have given. You can drill down to comparisons between the information you enter and the bacteria that appear to be the closest matches (which is mostly info from Bergeys, but is at least all in one place).

    • #95576
      Hmicro
      Participant

      wow what a wonderful website. Thank you sooooooo much!

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