Biology Forum › Microbiology › Using oxidizing agents in gram stain
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- September 22, 2013 at 2:44 pm #17533
ccBiologistParticipantConsidering the fact that Iodine is an oxidizing agent, what would happen if I replaced iodine with another oxidizing agent during the gram staining process ? So far I know that the purpose of the iodine is to form a complex with the crystal violet in order to enable the cell wall of the bacteria to retain the dye but certainly there must be other oxidizing agents that can replace iodine ?
- September 22, 2013 at 3:13 pm #114389CatParticipant
Interesting, logical, and probably possible. However, what is the point? Iodine is very cheap and non-hazardous, so, why would you want to change it?
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