Biology Forum Microbiology Using oxidizing agents in gram stain

last updated by Cat 9 years, 8 months ago
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    • #17533

      Considering the fact that Iodine is an oxidizing agent, what would happen if I replaced iodine with another oxidizing agent during the gram staining process ? So far I know that the purpose of the iodine is to form a complex with the crystal violet in order to enable the cell wall of the bacteria to retain the dye but certainly there must be other oxidizing agents that can replace iodine ?

    • #114389

      Interesting, logical, and probably possible. However, what is the point? Iodine is very cheap and non-hazardous, so, why would you want to change it?

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