I do want some of them to die. I am testing for TS mutants, to test the hypothesis that the translocon actively directs topology of membrane proteins or the conformation of secreted proteins. I am developing a library of temperature sensitive mutants in a haploid yeast strain harboring its only copy of sec61 on a plasmid which can be shuffled with a mutant library of plasmid borne sec61. I want to screen a large library of ts mutants en mass transformed with the library of mutant sec61 sequences to identify sec61-based suppressors. These suppressors, in the simplest scenario, function to facilitate folding of the unknown ts. If suppressors can be identified and their corresponding ts target genes isolated through a separate plasmid complementation screen, valuable insights might be gleaned about the language of topology and the machine that reads that language.